Forward and reverse primers diluted to working concentration 10µm working stocks are sufficient for most assays. The mix is optimized for sybr green re actions and contains sybr green i dye amplitaq gold dna polymerase dntps with dutp passive reference and optimized buffer components. Power sybr green pcr master mix contains all of the components excluding the template and primers for superior sybr green reagent based real time pcr.
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