P P65 Antibody Cell Signaling

Human mouse rat monkey.

P p65 antibody cell signaling.

Invitrogen anti phospho nfkb p65 ser529 polyclonal catalog 44 711g. Western blot analysis of extracts from hela cells transfected with nontargeted or targeted sirna. Phospho nf kappab p65 ser536 antibody detects nf kappab p65 only when phosphorylated at serine 536. Phospho nf κb p65 ser276 antibody detects nf κb p65 only when phosphorylated at ser276.

P50 and p65 form the most common heterodimer in the nf κb signaling pathway present in most cell types. Remaining members include relb c rel and cleaved proteins nf κb1 p50 and nf κb2 p52. Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding ser536 of human nf κb p65. P65 also known as rela is one of the five components that form the nf κb nuclear factor kappa light chain enhancer of activated b cells transcription factor family.

Western blot analysis of extracts from various cell lines using nf κb p65 d14e12 xp rabbit mab. Species predicted to react based on 100 sequence homology. P65 was detect using nf κb p65 antibody 3034 and p42 was detected using p42 mapk antibody 9108. The amount of immunoprecipitated dna in each sample is represented as signal relative to the total amount of input chromatin which is equivalent to one.

The absence of signal in the nf κb p65 knockout hela cells confirms the specificity of the antibody for nf κb p65. Human mouse rat monkey. Phospho nf kappab p65 ser536 antibody detects nf kappab p65 only when phosphorylated at serine 536. The inhibitory effect of i kappa b upon nf kappa b the cytoplasm is exerted primarily through the interaction with p65.

Western blot analysis of extracts from hela cells transfected with 100 nm signalsilence control sirna unconjugated 6568 or signalsilence nf κb p65 sirna i 6261 using nf κb p65 l8f6 mouse mab upper or α tubulin 11η10 rabbit mab 2125 lower. The antibody does not cross react with the p50 subunit or other related proteins. Supplied as 100 µl purified antibody. The antibody does not cross react with the p50 subunit or other related proteins.

The nf κb p65 antibody confirms silencing of p65 expression and the p42 mapk antibody is used to control for loading and sirna specificity. Species predicted to react based on 100 sequence homology. It also cross reacts with the homologous phosphorylated residues in c rel ser267 and nf κb1 p105 p50 ser337. This antibody reacts with human mouse samples.

Tested in western blot wb and immunohistochemistry paraffin ihc p applications.